新闻中心

Observing the cell's protein factories during self-assembly

作者:Charité - Universitätsmedizin Berlin

时间 :2018/6/13 0:00:00

Berlin-based researchers have produced snapshots of the 'protein factories' of the cell. Their findings could set us on the path towards a new class of antibiotics. The study - a basic science study conducted by researchers from Charité - Universitätsmedizin Berlin and the Max Planck Institute for Molecular Genetics - has been published in Molecular Cell*.

Ribosomes are the 'protein factories' of the cell and are composed of two units: a larger 50S subunit, and a smaller 30S subunit. The larger 50S subunit in turn comprises 33 different proteins and 2 ribonucleic acid molecules. The aim of the study was to obtain detailed information about the way in which these numerous different molecules form 50S subunits in bacteria. In a process known as 'in vitro reconstitution', the individual components were first purified and then mixed together in the laboratory. The researchers then observed how the 50S subunit assembled from this mixture. Using 3D cryo-electron microscopy, a technology whose developers received the 2017 Nobel Prize in Chemistry, they were able to produce high-resolution snapshots of individual stages of the 50S subunit's assembly and maturation. This allowed them to identify, on a molecular level, the individual steps involved in its development.

Explaining the significance of this study, Dr. Rainer Nikolay, of Charité's Institute of Medical Physics and Biophysics, comments: "These results do not simply provide us with important insights into one of the essential processes taking part inside the cell. They also provide us with information on potential targets for new antibacterial drugs." These could potentially inhibit ribosome assembly, thereby paralyzing the bacterium's ability to synthesize proteins; these drugs could therefore be used to inhibit all bacterial growth.

Dr. Nikolay plans to conduct additional research to test whether the process of 50S subunit assembly inside the living cell is the same as that observed outside the cell. He explains: "Much of the information available in the literature suggests that 50S assembly in vivo follows a very similar course to that observed in vitro. To test this assumption, we are currently developing methods capable of analyzing the structure of 50S ribosomal subunit precursors, which we will obtain directly from cells."

###

*Nikolay R, Hilal T, Qin B, Mielke T, Bürger J, Loerke J, Textoris-Taube K, Nierhaus KH, Spahn CMT. Structural visualization of the formation and activation of the 50S ribosomal subunit during in vitro reconstitution. Molecular Cell 2018. DOI: 10.1016/j.molcel.2018.05.003.

<< 上一篇:AHA wwards Cleveland Clinic $3.7 million for atrial fibrillation research 下一篇:Antarctic fungi found to be effective against citrus canker>>
技术支持:北京华宇星航国际教育科技有限公司 京ICP备18034610号

人工智能在线咨询

智能在线咨询 X

关于我们

该服务平台支持关键词检索、文献题名检索(准确题名)、文献DOI号检索、作者检索。当查询题名返回的结果无法下载全文数据或查询不到时,请找到所查文献DOI号后再次查询。查询结果会在10秒内返回, 24小时不间断的提供文献查询服务。

微信端

人工智能在线咨询